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Polyribosomes and protein synthesis in after‐ripening pear seeds

Identifieur interne : 002431 ( Istex/Checkpoint ); précédent : 002430; suivant : 002432

Polyribosomes and protein synthesis in after‐ripening pear seeds

Auteurs : Ruth Alscher-Herman [États-Unis] ; A. A. Khan [États-Unis]

Source :

RBID : ISTEX:12FF7F5DF678BFBF451EB1173F35E3568F5C486B

English descriptors

Abstract

Polyribosomes have been isolated from pear seeds which show a capacity for in vitro protein synthesis. Translational capacity increased during stratification with a peak after 13 days of stratification. This increase was greatly diminished in seeds which were incubated at 25°C (“warm stratification”). The peak in protein synthetic activity coincides with the period of stratification required for normal development of excised embryos.

Url:
DOI: 10.1111/j.1399-3054.1980.tb03256.x


Affiliations:


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ISTEX:12FF7F5DF678BFBF451EB1173F35E3568F5C486B

Le document en format XML

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<name sortKey="Alscher Erman, Ruth" sort="Alscher Erman, Ruth" uniqKey="Alscher Erman R" first="Ruth" last="Alscher-Herman">Ruth Alscher-Herman</name>
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<name sortKey="Khan, A A" sort="Khan, A A" uniqKey="Khan A" first="A. A." last="Khan">A. A. Khan</name>
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<wicri:cityArea>Department of Seed and Vegetable Sciences, New York State Agricultural Experiment Station, Cornell University, Geneva</wicri:cityArea>
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<title level="j" type="main">Physiologia Plantarum</title>
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<term>Apple seeds</term>
<term>Bottom figure</term>
<term>Cold treatment</term>
<term>Cornell university</term>
<term>Creatine phosphokinase</term>
<term>Embryo</term>
<term>Embryonal dormancy</term>
<term>Embryonic axis</term>
<term>Energy cocktail</term>
<term>Germinated</term>
<term>Germinated seeds</term>
<term>Germination</term>
<term>Germination conditions</term>
<term>Guinn assay</term>
<term>Intact pear seeds</term>
<term>Intact seeds</term>
<term>Isco density gradient fractionator</term>
<term>Lewak</term>
<term>Nucleic acids</term>
<term>Orcinol assay</term>
<term>Orcinol assays</term>
<term>Pear</term>
<term>Pear seed</term>
<term>Pear seeds</term>
<term>Petri dishes</term>
<term>Physiol</term>
<term>Plant physiol</term>
<term>Polyribosomes</term>
<term>Protein synthesis</term>
<term>Reaction mixture</term>
<term>Ribosome</term>
<term>Specific activity</term>
<term>Stratification</term>
<term>Stratification requirements</term>
<term>Stratified</term>
<term>Stratified seeds</term>
<term>Stratifying seeds</term>
<term>Sucrose</term>
<term>Sucrose density gradient</term>
<term>Sucrose density gradient centrifugation</term>
<term>Sucrose density gradients</term>
<term>Symmetric embryos</term>
<term>Symmetric growth</term>
<term>Time course</term>
<term>Translational</term>
<term>Translational activity</term>
<term>Translational capacity</term>
<term>Wheat germ</term>
<term>Wheat germ system</term>
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<div type="abstract" xml:lang="en">Polyribosomes have been isolated from pear seeds which show a capacity for in vitro protein synthesis. Translational capacity increased during stratification with a peak after 13 days of stratification. This increase was greatly diminished in seeds which were incubated at 25°C (“warm stratification”). The peak in protein synthetic activity coincides with the period of stratification required for normal development of excised embryos.</div>
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